Journal: Analytical Chemistry
Article Title: Electrophoresis: An Effective Viability Assessment Method across Cell Domains
doi: 10.1021/acs.analchem.5c05986
Figure Lengend Snippet: Separation between viable and nonviable eukaryotic cells. (a) Images of the viable and nonviable S. cerevisiae cells as they flow across the post array. (b) Electropherogram of the separation between viable and nonviable S. cerevisiae cells (ATCC 4098) with a resolution of Rs = 1.5 and Δ t R = 99 s, obtained at an average electric field of 55.5 V/cm in the post array. (c) Images of the viable and nonviable S. cerevisiae cells as they flow across the post array. (b) Electropherogram of the separation between viable and nonviable S. cerevisiae cells (ATCC 9080) with a resolution of Rs = 4.7 and Δ t R = 184 s, obtained at an average electric field of 99.4 V/cm in the post array. The electropherograms were obtained by employing the fluorescence signal from the cells as they cross the interrogation window indicated in b. The voltages employed in these separations are listed in . Videos of these separations are included in the Supporting Information as Videos S3 and S4 .
Article Snippet: The separations of viable and nonviable S. cerevisiae cells (ATCC 4098 and ATCC 9080, ) were conducted employing the same methodology and device design used with prokaryotic cells. a, b show the results of the separation of a mixture of viable and nonviable S. cerevisiae (ATCC 4098).
Techniques: Fluorescence